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| Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari. |
| The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577 |
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| Massucato, Luana Rainieri; Nakamura, Karina Kazue; Ruas, Paulo Mauricio; Zefa, Douglas Mariani; Silva, Derly José Henrique da; Gonçalves, Leandro Simões Azeredo. |
| The conservation of okra landraces [Abelmoschus esculentus (L.) Moench] in gene banks is essential for the success of their use in breeding programmes. This study evaluated the genetic diversity among okra landraces in Brazil based on morphoagronomic descriptors and AFLP markers. We studied 30 accessions of the vegetable gene bank of the Universidade Federal de Viçosa. To this end, 17 morphoagronomic descriptors and five combinations of AFLP primers were used. Genetic parameters were estimated for the quantitative traits and the accessions were grouped by Ward’s method, using the Gower’s and Jaccard’s distance measures, respectively, for the morphoagronomic and molecular data. Polymorphisms were observed for all qualitative traits, while the quantitative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Molecular markers; Phenotype; Bayesian analysis.; Recursos Genéticos. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43426 |
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